The Spatiotemporal Expression of Vegf in Fracture Healing
نویسندگان
چکیده
VEGF is a well known angiogenic peptide which has been shown to be central to endochondral ossification. Secondary fracture healing involves a combination of intramembranous and endochondral ossification. VEGF has been recently shown to be chemotactic for osteoblasts [1] and chondroclasts and central to endochondral ossification [2]. Fracture healing physiology involves a complex cascade of numerous factors. This study examined the temporal and spatial expression of VEGF in the standard closed rodent fracture model using immunohistochemistry and in situ hybridisation.
منابع مشابه
Heterologous expression of a truncated form of human recombinant vascular endothelial growth factor-A and its biological activity in wound healing
Objective(s): Vascular endothelial growth factor (VEGF) is one of the most effective proteins in angiogenesis, mesenchymal stem cells (MSCs) differentiation and wound healing. These abilities are therapeutic potential of VEGF in diabetic retinopathy, nephropathy and other tissue damage circumstances. In this study, recombinant VEGF was produced in Escherichia coli (E. coli) system and then biol...
متن کاملEvaluation of human platelet gel effect on production process and expression level of VEGF in experimental skin wounds in rat
Abstract Background and Objectives Platelet gel prepared from platelet-rich plasma, containing growth factors, accelerates reconstruction of skin layers, stimulation of angiogenesis and increase of collagen synthesis in wounds healing process. In this study, the effect of human platelet gel on was investigated VEGF expression in experimental skin wounds in rat. Materials and Methods In thi...
متن کاملSynergistic Effects of Graphene Oxide and Vascular Endothelial Growth Factor Immobilized in Polycaprolactone Nanofiber as a Candidate for Diabetic Wound Healing
Background & Objective: The combination of two or more therapeutic agents and their synergetic impacts can be therapeutically effective against multifactorial diseases, such as diabetic foot ulcers. This study demonstrates the application of a nanofiber-based drug delivery system with a controlled release of the growth factor. Various studies have shown that vascular endothelial growth facto...
متن کاملHydrogen sulfide improves vessel formation of the ischemic adductor muscle and wound healing in diabetic db/db mice
Objective(s): It has been demonstrated that hydrogen sulfide plays a vital role in physiological and pathological processes such as regulating inflammation, oxidative stress, and vessel relaxation. The aim of the study was to explore the effect of hydrogen sulfide on angiogenesis in the ischemic adductor muscles of type 2 diabetic db/db mice and ischemic diabetic wound...
متن کاملEffect of the timing of surgery on the fracture healing process and the expression levels of vascular endothelial growth factor and bone morphogenetic protein-2
The aim of the present study was to observe the effect of varying the timing of surgery on the fracture healing process and the expression levels of vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP)-2 in rats. A total of 192 rats underwent closed femur fracture modelling. The rats underwent open reduction and internal fixation surgery 1, 3, 5, 7, 11 and 14 days subs...
متن کامل